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alchemyTM HPLC Columns The Queen of Beauty in Chromatography A Major Advance in HPLC Columns through NanotechnologyalchemyTM HPLC columns are made of spherical silica particles manufactured of the highest purity (〉99.999%) in the market. The highest quality silica, an innovative surface nano-modification and unique bonding processes result in exceptional C18 phases with outstanding properties. One of the most remarkable properties of every alchemyTM column is that all types of organic compounds (neutral, basic or acidic) will have perfect peak symmetry and excellent efficiency across the whole applicable pH range. The proprietary nano-surface modification generates a very smooth and non-reactive surface, reducing the interaction between the silica surface and polar compounds, especially the basic compounds. Lot-to-lot reproducibility is ensured by a stable bonding/end-capping process that is extremely rugged. The uniform, spherical particles have a nominal surface area of 360 m2/g with a controlled pore size of 100Å. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, lifetime and column-to-column reproducibility.Unprecedented Experience in ChromatographyWith the alchemyTM columns, you will have an unprecedented experience in HPLC separation. Best peak symmetry and efficiency. This is one of the most remarkable features of our columns. They just outperform competitors’ products across the whole applicable pH range. Their peak shape and column efficiency are independent of pH. This provides maximum flexibility for method development and optimum ruggedness for the methods developed. Tightest specification. All our columns have tighter specification (see Table 2) than those of brand name manufacturers. This ensures that each column has great performance and identical se-lectivity. Best value. Because of our unique business structure and well-controlled processes, we are able to provide products of the highest quality at very competitive price; we are also able to customize any of our products based on customer requirements. Broad se-lection on prices and features. We offer a full line of products for a wide variety of applications and are willing to assist customers in optimizing their particular applications特征of alchemyTM columnsthe columns’用戶效果Perfect peak symmetry, independent of pH and mobile phase Highest flexibility for any application,Better sensitivity,Greater confidence on HPLC results Broad pH range (1-10.5)Flexibility for method developmentCompatible with 100% aqueous mobile phaseMaximum versatilityHighest surface coverageTolerance of large volume and mass injectionA wide variety of chemistriesBroad se-lection of polarity and Maximum versatility Minimum buffer concentration neededBetter sensitivity for LC/MS, extended column lifetime Tightest specificationBetter reproducibility, Greater confidence on the HPLC resultsExcellent column efficiencyBetter and faster separationGreat column lifetimeLower cost; greater convenience Comparison of features and specification Waters Symmetry-C184.6 x 150 mm, 5umZorbax XDB-C184.6 x 150 mm, 5um alchemy XBP-C184.6 x 150 mm, 5umSpec. on efficiencyna800011000Spec. on tailing factor (toluene) na0.98-1.250.96-1.10Spec. on tailing factor-amitriptyline1.45na (typically 1.5-2.0)1.25Spec. on se-lectivity(amitriptyline/acenaphthene)+/- 10%na+/- 7%pH range2-82-91.5-10.5Specific Surface 300180360Carbon%16%12%22%Inertness and efficiency comparison Sample: uracil, toluene, doxepin , amitriptyline: Column: 4.6 x 150, 5 umMobile phase: 10 mM sodium phosphate (pH=7) in 60% ACN.Temperature: 30 oC; Flow(: 1 mL/min. The plate numbers in regular font are for toluene, the plate numbers in bold are for amitriptyline Volume Loading Capacity Comparison Large Volume Injection (injection volume =100 uL, ) Sample(: 4-methoxybenzenesulfonamide, 10 ug/mL in MeOH/Water (1:1)色譜柱: 4.6 x 150 mm, 5um particle sizeMobile phase: MeOH/WaterTemperature: 30 oCFlow: 1mL/min.Mass Load Comparison Columns(: 21.2 x 150 mm, C18, 5um Sample: diphenhydramine, 100 mg/mL in DMSO, 800uL Mobile phase: 65% 10 mM ammonia: 35% ACN A Wide Selection of alchemy TM ColumnsTo meet differing application needs with-in the corresponding pH requirements, we have the following offerings available in our reverse phase line-up:alchemyTM XBP series, XBP-C18 and XBP-C8, are bonded to the maximum density to have the highest hydr-ophobicity (the lowest polarity), the lowest interaction of analytes with silanols, high stability at high pH and the broadest pH range. alchemyTM XBP系列,XBP-C18和XBP-C8, alchemyTM AQ-C18 columns are more polar than XBP and have more separation power for polar compounds. Due to special silica treatment, they are true C18 columns that are compatible with 100% aqueous mobile phase. Like XBP columns, they are still very friendly towards those very basic analytes. Although their upper pH limit is not as high as XBP, it is comparable to many popular brand name columns. alchemyTM LP-C18 columns are the most polar among C18 columns due to a polar end-cap and are preferred replacement for Waters Atlantis and Zorbax SB-C18. They are extremely stable at low pH and may also be used at mid-pH. Unlike Atlantis and SB-C18, LP-C18 columns can still maintain perfect peak symmetry even for very basic analytes and are compatible with 100% aqueous mobile phases.在 C18 columns中alchemyTM LP-C18 columns Comparison of alchemyTM -C18 PhasesXBP-C18AQ-C18LP-C18Surface area (m2/g)360360360C%22%18%17%End-capdoublesinglepolarPolarityIncrease àpH stability1.5-10.51.5-91-7100% water compatible noyesyesPeak shape of bases excellentexcellentexcellentReplacedcolumnsZobax XDBSymmetryXterraLunaDiscoverySymmetryLuna (2)Inertsil ODS-3AQ phasesZorbax SB-C18Zorbax SB-AQWaters AtlantisAQ phases Use of organic base for pH 〉 9 is recommended . Test conditions:columns:2.1 x 50 mm, 5um;mobile phase: 60% or 65% MeOH in water;flow:0.2 mL/mintemperature: 30 oCinjection:1uLsample:uracil, phenol, chloronitrobenzene (CNB), toluene (TOL)detection:UV 254 nm alchemy XBP Columns alchemyTM XBP columns, XBP-C18 and XBP-C8, are bonded to the maximum density to have the highest hydr-ophobicity (the lowest polarity), the lowest interaction of analytes with silanol, high stability at high pH and the broadest pH range. Pore size: 100 A; Specific surface: 360 m2/g; Purity 〉 99.999%; 1)Inertness: one of most base-friendly columns.2)Efficiency: the best for basic compounds at any applicable pH.3)Large volume injection: maintain very high efficiency even if the injection volume is exceptional large.4)Broad pH range (1.5 – 10.5 ): Low pH, 0.2 % TFA; High pH, 0.010 M triethylamine.Column packing quality Sample: uracil, phenol, chloronitrobenzene, toluene (in methanol/water=50/50)Columns: 5 um, 2.1 x 50 mmMobile phase: methanol/water (65/35)Temperature: 30 oC Flow: 0.20 mL/minPlates (toluene)Tailing FactorZorbax XDB-C836051.18Luna C825461.15Venusil XBP-C8 40121.03 Great stability at low pH Column: Venusil XBP –C18, 4.6 x 150 mm, 5 umAging: 40 oC, pH= 1.0, TFA in 80 % Methanol, 400 hours, Flow= 1.0 mL/minTesting: 30 oC, pH=1.5, TFA in 80% methanol, 5 uL inj. of naphthlenePerfect peak symmetry and great lifetime of the column for basic compounds at mid-pH Sample: Uracil, Doxepin, Nortriptyline, Amitriptyline, TrimipramineColumn: Venusil XBP-C18, 4.6 x 150 mm, Mobile phase: 0.01 M sodium phosphate/ACN (50/50), pH=7.6Flow: 1.5 mL/minTemperature: 45oC Ordering InformationAnalytical alchemyXBP-C18 ColumnsParticle Size 5µm2.1mm ID cat.#4.6mm IDcat.#30mm LengthVX950302-0VX950305-0245.00260.0050mm LengthVX950502-0VX950505-0245.00270.00100mm LengthVX951002-0VX951005-0295.00320.00150mm LengthVX951502-0VX951505-0335.00360.00250mm LengthVX952502-0VX952505-0inquire375.00Particle Size 3µm2.1mm ID cat.#4.6mm ID cat.#30mm LengthVX930302-0VX930305-0260.00285.0050mm LengthVX930502-0VX930505-0260.00315.00100mm LengthVX931002-0VX931005-0295.00375.00150mm LengthVX931502-0VX931505-0355.00389.00Analyticalalchemy XBP-C8 ColumnsParticle Size 5µm2.1mm ID cat.#4.6mm ID cat.#30mm LengthVX850302-0VX850305-0245.00260.0050mm LengthVX850502-0VX850505-0245.00270.00100mm LengthVX851002-0VX851005-0295.00320.00150mm LengthVX851502-0VX851505-0335.00360.00250mm LengthVX852502-0VX852505-0inquire375.00Preparative alchemy XBP-C18 ColumnsParticle Size 10µm20mm ID cat.#30mm ID cat.#50mm LengthVX900520-0VX900530-0795.00inquire100mm LengthVX901020-0VX901030-0980.002200.00150mm LengthVX901520-0VX901530-01180.002850.00250mm LengthVX902520-0VX902530-01590.003490.00Particle Size 5µm20mm ID cat.#30mm ID cat.#50mm LengthVX950520-0VX950530-0895.00inquire100mm LengthVX951020-0VX951030-0995.002290.00150mm LengthVX951520-0VX951530-01380.002950.00250mm LengthVX952520-0VX952530-01890.003890.00 alchemy AQ C18 Columns alchemyTM AQ-C18 columns are designed for separation of polar and semi-polar compounds at low to medium pH. They are more polar than XBP-C18 and less polar than LP-C18. They are true C18 columns. However, due to a special surface treatment , they are compatible with 100% aqueous mobile phase. They are stable at low pH 1.5 – 9.0 and are preferred replacement for Waters Symmetry, Inertsil C18, Zorbax SB, and many AQ columns.Pore size: 100 A; Specific surface: 360 m2/g; Purity 〉 99.999%; 1)Inertness: One of most base-friendly columns, excellent peak symmetry of basic compounds. 1)Efficiency: best for polar compounds at any pH.1)Large volume injection: Maintains very high efficiency even if the injection volume is exceptional large.1)100% water compatible: Very unique for a universal C18 column; far better peak shape, retention and efficiency than those so-called high aqueous “AQ” columns. 1)Low pH Stability ( pH=1.5 ): Better stability than most of popular brand columns on the market and much more stable than most of AQ columns .Perfect peak symmetry and good stability at med-pH for basic compounds Sample: Uracil, Doxepin, Nortriptyline, Amitriptyline, TrimipramineColumn: alchemy AQ-C18, 4.6 x 250 mm, Mobile phase: 0.01 M sodium phosphate/ACN (25/75), pH=7.0Flow: 1.3 mL/min. Temperature: 30oC Compatibility with 100% aqueous mobile phase Sample: UridineColumn: 4.6 x 150 mmMobile phase: 100% waterFlow: 1mL/min.; the flow was stopped for 5 minutes during the testing period for each column.Temperature: 30 oCStability of alchemy AQ-C18 at pH=1.5 Sample: Uracil, Doxepin, Amitriptyline, Toluene.Column: alchemy AQ C18, 4.6 x 150 mm, 5 umMobile phase: TFA (0.25%, pH=1.5) in 60% acetonitrileFlow: 1 mL/ min.; Temp: 40 oC Ordering InformationAnalytical alchemyAQ-C18 ColumnsParticle Size 5µm2.1mm ID cat.#4.6mm ID cat.#30mm LengthVA950302-0VA950305-0245.00260.0050 mm LengthVA950502-0VA950505-0270.00285.00100mm LengthVA951002-0VA951005-0295.00320.00150mm LengthVA951502-0VA951505-0335.00370.00250mm LengthVA952502-0VA952505-0inquire390.00Particle Size 3µm2.1mm ID cat.#4.6mm ID cat.#30mm LengthVA930302-0VA930305-0260.00285.0050mm LengthVA930502-0VA930505-0260.00315.00100mm LengthVA931002-0VA931005-0295.00375.00150mm LengthVA931502-0VA931505-0355.00389.00Preparative alchemy AQ-C18 ColumnsParticle Size 10µm20mm ID cat.#30mm ID cat.#50mm LengthVA900520-0VA900530-0920.001980.00100mm LengthVA901020-0VA901030-01090.002200.00150mm LengthVA901520-0VA901530-01190.002500.00250mm LengthVA902520-0VA902530-01590.003890.00 alchemy LP Columns alchemy LP columns are specially designed for separation of polar compounds at very low to medium pH. They are end-capped with polar groups and have the highest polarity among Venusil columns. They are extremely stable at low pH (pH= 1) and are preferred replacement for Waters Atlantis, Zorbax SB, and many AQ columns.Pore size: 100 A; Specific surface: 360 m2/g; Purity 〉 99.999%; 1Low pH Stability ( pH=1): it has the best stability at low pH2Peak shape and efficiency: Unlike many polar phase columns, it has excellent peak symmetry of basic compounds.3Polar C18 phase very strong separating power for polar compounds4Large volume injection: maintain very high efficiency even if the injection volume is exceptional large.5100% water compatible: better peak shape, retention and efficiency than those so-called high aqueous “AQ” columns. Ordering InformationAnalytical alchemy LP-C18 ColumnsParticle Size 5µm2.1mm IDCat. # 4.6mm ID Cat. # 30mm LengthVL950302-0VL950305-0245.00260.0050mm LengthVL950502-0VL950505-0270.00285.00100mm LengthVL951002-0VL951005-0295.00320.00150mm LengthVL951502-0VL951505-0335.00370.00250mm LengthVL952502-0VL952505-0355.00390.00Particle Size 3µm2.1mm IDCat. # 4.6mm ID Cat. #30mm LengthVL930302-0VL930305-0260.00285.0050mm LengthVL930502-0VL930505-0260.00315.00100mm LengthVL931002-0VL931005-0295.00375.00150mm LengthVL931502-0VL931505-0355.00389.00